Size: 10 reactions.
pEASY®-Blunt M2 Expression Vector utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector under regulation of enhanced CMV promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
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• 5 minutes fast ligation of Pfu-amplified PCR products.
• Enhanced CMV promoter for higher protein yield.
• CMV forward primer and TK polyA reverse primer for sequencing.
• C-terminal myc tag for protein detection.
• Neomycin resistance gene for stable cell line selection.
• M2 Expression Plasmid included as negative control.
Chemically Competent Cell at -70°C for six months; others at -20°C for nine months
CM211-01 (10 rxns)
pEASY ®-Blunt M2 Expression Vector (15 ng/μl)
M2 Expression Plasmid (Negative Control) (15 ng/μl)
MControl Template (5 ng/μl)
MControl Forward Primers (10 μM)
MControl Reverse Primers (10 μM)
CMV Forward Primer (10 μM)
TK PolyA Reverse Primer (10 μM)
Trans1-T1 Phage Resistant
Chemically Competent Cell
Papers citing the use of this product:
|The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer||Oncotarget||5.008||Du G, et al. Department of Biochemistry and Molecu||2016 May||The original link|
|A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression||anticancer research||1.895||Yang X, et al. Department of Biochemistry and Mole||2017 Jan||The original link|
|A Novel NHERF1 Mutation in Human Breast Cancer Inactivates Inhibition by NHERF1 Protein in EGFR Signaling||Anticancer Research||1.826||GUIFANG DU, et al.||2016 Mar||The original link|