pEASY®-Blunt M2 Expression Kit


Size: 10 reactions.

pEASY®-Blunt M2 Expression Vector utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector under regulation of enhanced CMV promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

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• 5 minutes fast ligation of Pfu-amplified PCR products.

• Enhanced CMV promoter for higher protein yield.

• CMV forward primer and TK polyA reverse primer for sequencing.

• C-terminal myc tag for protein detection.

• Neomycin resistance gene for stable cell line selection.

  • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• M2 Expression Plasmid included as negative control.


Chemically Competent Cell at -70°C for six months; others at -20°C for nine months

Kit Content:


CM211-01 (10 rxns)

pEASY ®-Blunt M2 Expression Vector (15 ng/μl)

10 µl

M2 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

MControl Template (5 ng/μl)

10 µl

MControl Forward Primers (10 μM)

10 µl

MControl Reverse Primers (10 μM)

10 µl

CMV Forward Primer (10 μM)

50 µl

TK PolyA Reverse Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant

5×100 μl

Chemically Competent Cell

Papers citing the use of this product: 

The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancerOncotarget5.008Du G, et al. Department of Biochemistry and Molecu2016 MayThe original link
A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progressionanticancer research1.895Yang X, et al. Department of Biochemistry and Mole2017 JanThe original link
A Novel NHERF1 Mutation in Human Breast Cancer Inactivates Inhibition by NHERF1 Protein in EGFR SignalingAnticancer Research1.826GUIFANG DU, et al.2016 MarThe original link

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Blunt M2 expression kit

pEASY®-Blunt M2 Expression Kit

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