Transetta(DE3) Chemically Competent Cell

Transetta(DE3) Chemically Competent Cell is specifically designed from imported strain for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype: F- ompT hsdSB(rB- mB-) gal dcm lacY1(DE3)pRARE(argU, argW, ileX, glyT, leuW, proL)(Camr) 

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  • 20 reactions
  • 40 reactions

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• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• CamR.

• tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA. Enhance the expression level of proteins in the prokaryotic system.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.


at -70°C for six months. Do not store in liquid nitrogen. 

Papers citing the use of this product:

Exploring the Catalytic Promiscuity of a New Glycosyltransferase from Carthamus tinctoriusOrganic Letters6.2Kebo Xie?? et al2014 SepThe original link
Mesomartoxin, a new Kv1.2-selective scorpion toxin interacting with the channel selectivity filterBiochemical Pharmacology5.009Xueli Wang, et al.2014 DecThe original link
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional GenesFRONTIERS IN PLANT SCIENCE4.2979Zhiyuan Liu,et al.2017 AugThe original link
The Divergence of Flowering Time Modulated by FT/TFL1 Is Independent to Their Interaction and Binding ActivitiesFRONTIERS IN PLANT SCIENCE4.29Zhen Wang et al Key Laboratory of Horticultural Pl2017 MayThe original link
Combined Virtual Screening and Substructure Search for Discovery of Novel FABP4 Inhibitorsjournal of chemical information and modeling3.7599Haiyan Cai,et al.2017 AugThe original link

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Transetta cells

Transetta(DE3) Chemically Competent Cell

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