pEASY®-Blunt E2 Expression Kit

• 5 minutes fast ligation of Pfu-amplified PCR products.

• Ampicillin resistance.

• T7 promoter primer and T7 terminator primer for sequencing.

• Bacteriophage T7lac promoter for high level expression.

• C-terminal 6×His tag for easy purification.

• Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

• E2 Expression Plasmid included as negative control.

Storage

Chemically Competent Cell at -70°C for six months; others at -20°C for nine months

Kit Content

Component

CE211-01 (10 rxns)

pEASY®-Blunt E2 Expression Vector (15 ng/μl)

10 µl

E2 Expression Plasmid (Negative Control) (15 ng/μl)

10 µl

EControl Template (5 ng/μl)

10 µl

EControl Forward Primer (10 μM)

10 µl

EControl Reverse Primer (10 μM)

10 µl

T7 Promoter Primer (10 μM)

50 µl

T7 Terminator Primer (10 μM)

50 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl