Fast MultiSite Mutagenesis System


Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.

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• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.

• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.

• Efficient: >90% mutagenesis efficiency. 


We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.


at -20°C for two years

Kit Content


FM201-01 (10 rxns)

TransStart® FastPfu PCR SuperMix

1 ml

DMT Enzyme (10 units/μl)

30 μl

2×Assembly Mix

50 μl

DMT Chemically Competent Cell

10×50 μl


1 ml

Papers citing this product:

Regulation of Antimicrobial Peptides in Aedes aegypti Aag2 CellsFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY5.218Zhang R, et al. Tsinghua-Peking Center for Life Sc2017 FebThe original link
The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancerOncotarget5.008Du G, et al. Department of Biochemistry and Molecu2016 MayThe original link
Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directedmutagenesisapplied microbiology and biotechnology3.376Chen Y, et al. The Key Laboratory of Industrial Bi2016 NobThe original link
Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behaviorAmino Acids3.173Fa?Min Zeng,et al.2017MayThe original link
Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteriabioscience reports2.9059Yu-Xing Lu,et al.2017JulThe original link

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Fast MultiSite Mut Sys

Fast MultiSite Mutagenesis System

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