Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.
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• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.
at -20°C for two years
FM201-01 (10 rxns)
2×TransStart® FastPfu PCR SuperMix
DMT Enzyme (10 units/μl)
DMT Chemically Competent Cell
Papers citing this product:
|Regulation of Antimicrobial Peptides in Aedes aegypti Aag2 Cells||FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY||5.218||Zhang R, et al. Tsinghua-Peking Center for Life Sc||2017 Feb||The original link|
|The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer||Oncotarget||5.008||Du G, et al. Department of Biochemistry and Molecu||2016 May||The original link|
|Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directedmutagenesis||applied microbiology and biotechnology||3.376||Chen Y, et al. The Key Laboratory of Industrial Bi||2016 Nob||The original link|
|Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior||Amino Acids||3.173||Fa?Min Zeng,et al.||2017May||The original link|
|Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteria||bioscience reports||2.9059||Yu-Xing Lu,et al.||2017Jul||The original link|