pEASY®-T5 Zero Cloning Kit

pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.

•5 minutes fast ligation.

•High cloning efficiency. Positive clones up to 100%.

•No blue/white selection needed.

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CAD$377.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.

•5 minutes fast ligation of Taq-amplified PCR products.

•High cloning efficiency. Positive clones up to 100%.

•No blue/white selection needed.

•Suitable for short and large fragment cloning.

•Kanamycin and Ampicillin resistance genes for selection.

•M13 forward primer and M13 reverse primer for sequencing.

•T3 promoter and T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CT501-01

CT501-02

(20 rxns)

(60 rxns)

pEASY ®- T5 Zero Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans 1-T1 Phage Resistant

10×100 μl

30×100 μl

Chemically Competent Cell

References:

LiteratureJournalIFAuthorDateLink
Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scaleNATURE METHODS25.328Bo Xia, et al. State Key Laboratory of Protein and2015 SepThe original link
Enhanced Telomere Rejuvenation in Pluripotent Cells Reprogrammed via Nuclear Transfer Relative to Induced Pluripotent Stem CellsCell Stem Cell25.315Rongrong Le et al.2014 JanThe original link
Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in ReprogrammingCell Stem Cell25.315Yawei Gao, et al.2013 MarThe original link
Unique features of mutations revealed by sequentially reprogrammed induced pluripotent stem cellsNature Communications11.329Shuai Gao, et al. Ministry of Agriculture Key Labo2015 FebThe original link
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cellsCell Research10.526Gang Chang,et al.2013 DecThe original link

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