pEASY®-T1 Simple Cloning Kit

pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.

•5 min. fast ligation

•choice of resistance genes

•Blue/white selectio

•SR primer and M13 forward primer for sequencing

Trans1-T1 Phage resistant Competent Cells, high efficiency (>10^9 cfu/μg pUC19 DNA), fast growth rate.

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CAD$296.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•SR primer and M13 forward primer for sequencing.

•T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CT111-01

CT111-02

(20 rxns)

(60 rxns)

pEASY ®-T1 Simple Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13Forward Primer (10 μM)

50 µl

150 µl

M13Reverse Primer (10 μM)

50 µl

150 µl

SR Primer (10 μM)

50 µl

150 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

10×100 μl

30×100 μl

References:

LiteratureJournalIFAuthorDateLink
A transgenic strategy for controlling plant bugs(Adelphocoris suturalis) through expression of double-stranded RNA homologous to fatty acyl-coenzyme A reductase in cottonnew phytologist7.21Jing Luo,et al.2017JunThe original link
A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maizenew phytologist7.2Chao Wang et al National Maize Improvement Centre2017 JulThe original link
Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplificationanalytical chemistry6.32Yi Hu,et al.2016NovThe original link
Nitrogen deposition mediates the effects and importance of chance in changing biodiversityMolecular Ecology6.275Ximei Zhang, et al. State Key Laboratory of Vegeta2011 JanThe original link
Characterization of Cyclooxygenase-2 and its induction pathways in response to high lipid diet-induced inflammation in Larmichthys croceaScientific Reports5.578Tianjiao Wang, et al.2016 FebThe original link

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