pEASY®-Blunt Cloning Kit

pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•Trans1-T1 Phage Resistant Chemical Competent Cell, high efficiency (>10^9 cfu/μg pUC19 DNA) and fast growth rate.

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CAD$296.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CB101-01

CB101-02

(20 rxns)

(60 rxns)

pEASY ®- Blunt Cloning Vector (10 ng/μl)

20µl

3×20 µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

Trans1-T1 Phage Resistant

10×100 μl

30×100μl

Chemically Competent Cell

References

LiteratureJournalIFAuthorDateLink
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildewNature Biotechnology39.08Yanpeng Wang, et al.2014 JulThe original link
D14?CSCFD3-dependent degradation of D53 regulates strigolactone signalingNature38.597Feng Zhou,et al.2013 DecThe original link
??Perfect?? designer chromosome V and behavior of a ring derivativeScience34.661Xie Z, et al. Key Laboratory of Systems Bioenginee2017 MarThe original link
Targeted genome modification of crop plants using a CRISPR-Cas systemNature Biotechnology32.44Qiwei Shan,et al.2013 AugThe original link
Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compoundsCell Research12.413Junqing Ye, et al.2015 DecThe original link

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