Trans110 Chemically Competent Cell

Trans110 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^6 cfu/μg DNA (tested by pUC19 plasmid DNA), featuring

• High transformation efficiency: >10^6 cfu/μg ( pUC19 DNA).

• Unmethylated DNA due to dam-/dcm-.

• StrR.

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CAD$234.00

  • 20 reactions
  • 40 reactions

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Description

Trans110 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 106 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype

rpsL (StrR) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) /F′ [traD36proAB lacIq lacZΔM15]

Features

• High transformation efficiency: >106 cfu/μg ( pUC19 DNA).

• Unmethylated DNA due to dam-/dcm-.

• StrR.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.


References:

LiteratureJournalIFAuthorDateLink
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback mothPLoS Genetics8.167Zhaojiang Guo?? et al2015 AprThe original link
Bacillus thuringiensis subsp. sichuansis strain MC28 produces a novel crystal protein with activity against Culex quinquefasciatus larvaeWorld Journal of Microbiology and Biotechnology1.262Peng Guan, et al. State Key Laboratory of Hybrid R2014 AprThe original link
Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strainBiocontrol Science and Technology0.848Yu Z, et al. Sichuan agricultural university2015The original link

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