pEASY®-Blunt Simple Cloning Kit

pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning Pfu-amplified PCR products.

•5 min. fast ligation

•choice of resistance genes markers

•Blue/white selection

•SR primer, M13 forward primer for sequencing.

Trans1-T1 Phage Resistant Competent Cell, high efficiency (>10^9 cfu/μg pUC19 DNA) and fast growth rate.

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CAD$296.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.

•5 minutes fast ligation of Pfu-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•SR primer and M13 forward primer for sequencing.

•T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for one year

Kit Content

Component

CB111-01

CB111-02

(20 rxns)

(60 rxns)

pEASY ®-Blunt Simple Cloning Vector (10 ng/μl)

20µl

3×20µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

SR Primer (10 μM)

50µl

150µl

Trans1-T1 Phage Resistant Chemically Competent Cell

10×100µl

30×100µl


References:

LiteratureJournalIFAuthorDateLink
Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysisFungal Diversity5.319Yu Z, et al. Marine Biotechnology Laboratory, Stat2013 JanThe original link
Two Novel Fungal Phenolic UDP Glycosyltransferases from Absidia coerulea and Rhizopus japonicusapplied and environmental microbiology3.8229Kebo Xie,et al.2017SepThe original link
RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid OrganogenesisPLOS ONE3.73Bin Gao,et al.2013 AugThe original link
Polymorphisms of Chicken TLR3 and 7 in Different BreedsPLOS ONE3.73Wenke Ruan2015 MarThe original link
Molecular cloning and functional characterization of multiple NADPH-cytochrome P450 reductases from Andrographis paniculata.international journal of biological macromolecules3.6Huixin Lin et al National Resource Center for Chin2017 AprThe original link

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