Trans2-Blue Chemically Competent Cell

Trans2-Blue Chemically Competent Cells are for chemical transformation of DNA. It permits a transformation efficiency of over 10^9 cfu/μg DNA (tested by pUC19 plasmid DNA). The cells areresistant to tetracycline (TetR) and chloramphenicol (CamR), featuring: /High efficiency (>1×10^9cfu/μg DNA)/For larger plasmids and recombinant products/library construction/Blue/White selection.

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CAD$234.00

  • 20 reactions
  • 40 reactions

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Description

Trans2-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR) and chloramphenicol (CamR).

Genotype

TetRΔ(mcrA)183Hte[F′{proAB lacIq lacZΔM15Tn10(TetR)AmyCamR}]Δ(mcrCB-hsdSMR-mrr)173 endA1supE44 thi-1 recA1 gyrA96 relA1

Features

• High transformation efficiency (>1×109cfu/μg DNA).

• Suitable for larger plasmids and recombinant products.

• Reduced preference for plasmid size, suitable for library construction.

• Used in Blue/White selection.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure

References:

LiteratureJournalIFAuthorDateLink
Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucataPLoS One3.5328Fang D, et al. Institute of Marine Biotechnology,2011The original link
Characterization of an insect heterodimeric voltage-gated sodium channel with unique alternative splicing modecomparative biochemistry and physiology b-biochemi1.651Jiang XZ, et al. Key Laboratory of Entomology and2017 JanThe original link

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