TransDetect® Cell Counting Kit (CCK) is designed for cell proliferation assays as well as cytotoxicity assays by utilizing a water-soluble tetrazolium salt. The salt can be reduced to an orange water-soluble formazan by mitochondrial dehydrogenase in the presence of an electron coupling reagent 1-Methoxy PMS. The amount of the formazan dye formed is measured to reflect the number of living cells.
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The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. Faster cell proliferation, lower cytotoxicity, and more cell number produce deeper color. The depth of the dye is directly proportional to the number of living cells. The toxicity of CCK solution is so low that the same cells can be used for other assays after the CCK assay is completed. Compared with MTT, XTT, MST and WST-1, this method provides higher sensitivity and broader linear range. It is suitable for drug screening, cell proliferation test, cytotoxicity assay and drug sensitivity test.
at 2-8°C in dark for one year or at -20°C in dark for two years
Literature Citing this product
|Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion（doi: 10.1093/nar/gkx178）||nucleic acids research||9.202||Li P, et al. Institute of Virology and AIDS Resear||2017 Mar||The original link|
|GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer||cancer letters||5.992||Gu J, et al. Department of General Surgery, Ruijin||2016 Oct||The original link|
|A novel approach to prepare a tissue engineering decellularized valve scaffold with poly(ethylene glycol)–poly(ε-caprolactone)||RSC Advances||3.84||Zhigang Zhu, et al.||2016 Jan||The original link|
|LncRNA UCA1 promotes renal cell carcinoma proliferation through epigenetically repressing p21 expression and negatively regulating miR-495||Tumor biology||3.65||Yun Lu et al Department of Nephrology, The Affilia||2017 May||The original link|
|FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells||applied microbiology and biotechnology||3.42||Shuying Li,et al.||2017Apr||The original link|