Annexin V-FITC Apoptosis Detection Kit (with PI)

this kit provides reagents and protocols to detect apoptosis in cells using FITC-labelled Annexin V to stain cells with green fluorescence and using  propidium iodide to stain cells with red fluorescence. The kit can be used in Flow Cytometry, Fluorescence Microscope and other procedures. 

More details

CAD$150.00

  • 50 reactions
  • 100 reactions

More info

In healthy cells, phosphatidylserine (PS) only distributes in the inner layer of the cell membrane lipid bilayer. At the early stage of apoptosis, PS is turned from the inside of the lipid membrane to the outside. Annexin V, a Ca2+ dependent phospholipid binding protein with a molecular weight of 35-36 kD, has a high affinity to PS and can be used to detect to detect the externalization of PS. Annexin V is recognized as one of the sensitive indicators of early apoptosis. Apoptosis can be detected by fluorescence microscopy or flow cytometry using Annexin V probe conjugated to FITC (a green fluorescent). This kit also includes propidium iodide (PI), a dye for nucleic acid that cannot penetrate the intact cell membrane of healthy or early apoptotic cells. PI can penetrate the membrane of late apoptotic and necrotic cells, and stain these cells with red fluorescence. Using both Annexin V and PI, this kit can used to distinguish cells at different apoptotic periods, i.e. live cells (Annexin V-/PI-), early apoptotic cells (Annexin V+/PI-), and late apoptotic/dead cells (Annexin V+/PI+).

Component

A211-01 (50 rxn)

 A211-02 (100 rxn)

Annexin V-FITC

250 µl

500 µl

PI Staining Solution

250 µl

500 µl

1× Binding Buffer25 ml× 25 ml

 

Store all components at 4-8℃ and protect from light.
The Annexin V-FITC can be stored at -20℃ for longer use.

Fig. 1. Detection of TRAIL-induced apoptosis of Jurkat cells by flow cytometry (A) 0 ng/ml. (B) 0.5 ng/ml. (C) 1.5 ng/ml. (D) 4.5 ng/ml. (E) 13.5 ng/ml. (F) Jurkat cells were induced apoptosis for 3 hours by 13.5 ng/ml TRAIL and then treated with 10 μg unlabeled Annexin V for 10 min, followed by Annexin V-FITC/PI staining. Unlabeled Annexin V, competiting with Annexin V-FITC, was used to confirm the specificity of staining

6 other products in the same category:

Download