Long PCR

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Long PCR is PCR of long or large ragments, often also referred to as long-range PCR. In order to perform long PCR, not only the cycling times need to be increased, but also the polymerases need to be modified. Specific formulations with Taq DNA polymerase spiked with a low amount of proof-reading enzymes such as Pfu DNA polymerases are often used (W M Barnes (1994) Proc Natl Acad Sci U S A. 91(6): 2216–2220.)

 

Long PCR There are 5 products.

Showing 1 - 5 of 5 items
  • CAD$110.00

    GB-AMP™ PaCeR™ HP™ DNA Polymerase is a superior performance, highly flexible enzyme for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*.  This product is also capable of amplifying long fragments such as 20 kb genomic DNA and 40 kb λDNA.  View the PaCeR Brochure Here. 

    CAD$110.00
  • CAD$105.00

    A proven robust PCR enzyme, GB-AMP™ HP™ PaCeR™ DNA Polymerase is suitable for all types of PCR, i.e., High Fidelity, Long PCR, high GC/complex targets, Fast PCR, Direct PCR, as well as routine PCR, you have found it in this versatile and robust PCR reagent. It was compared to Phusion (Thermo) and 8 other enzymes and found to be the best all-round PCR DNA...

    CAD$105.00
  • CAD$70.00

    Master mix Long PCR Taq DNA Polymerase, the same high-performing polymerases, now in the convenient master mix format. The master mix is a special formulation containing the Long PCR Taq DNA polymerase. The Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such...

    CAD$70.00
  • CAD$80.00

    Long PCR Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. The Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.

    CAD$80.00
  • CAD$300.00

    This product is a 2X Long PCR reagent Mix, plus a separately supplied Long PCR DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations. This Optimzer format provides flexibility of optimizing Long DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes, such that long targets up to...

    CAD$300.00
Showing 1 - 5 of 5 items