PCR for Difficult Targets

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Usually after a routine PCR attempt, researchers would find that their PCR has failed to amplify the intended target into the correct product. The reasons for this failure can be a few, top on the list, is the complexity of PCR target. Specifically, when the target sequence, or the primer sequence, contains high GC content >60%, or if the target sequence contains a secondary structure, the targets are difficult to denature, leading to failed PCR. The solution to this problem is to use the PCR for Difficult Targets. This type of PCR is featured by using polymerases that are better able to handle the high complexity targets, or additives such as the PCR Enhancing Reagent. 

PCR for Difficult Targets There are 6 products.

Showing 1 - 6 of 6 items
  • CAD$100.00

    2X Achiever™ Taq Master Mix features Achiever DNA Polymerase, a mixture of Taq and Pfu polymerases, which blends the processivity of Taq with the high fidelity of Pfu. Taq Plus allows amplification of longer templates, and complex targets (e.g., GC-rich) with higher fidelity and than the single-enzyme formulations. 2X Achiever™ Taq Master Mix is also...

    CAD$100.00
  • CAD$100.00

    2X Achiever™ Taq Master Mix features Achiever DNA Polymerase, a mixture of Taq and Pfu polymerases, which blends the processivity of Taq with the high fidelity of Pfu. Taq Plus allows amplification of longer templates, and complex targets (e.g., GC-rich) with higher fidelity and than the single-enzyme formulations. 2X Achiever™ Taq Master Mix is also...

    CAD$100.00
  • CAD$105.00

    A proven robust PCR enzyme, GB-AMP™ HP™ PaCeR™ DNA Polymerase is suitable for all types of PCR, i.e., High Fidelity, Long PCR, high GC/complex targets, Fast PCR, Direct PCR, as well as routine PCR, you have found it in this versatile and robust PCR reagent. It was compared to Phusion (Thermo) and 8 other enzymes and found to be the best all-round PCR DNA...

    CAD$105.00
  • CAD$110.00

    GB-AMP™ PaCeR™ HP™ DNA Polymerase is a superior performance, highly flexible enzyme for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*.  This product is also capable of amplifying long fragments such as 20 kb genomic DNA and 40 kb λDNA.  View the PaCeR Brochure Here. 

    CAD$110.00
  • CAD$30.00

    PCR Enhancing Reagent can be used to improve PCR of difficult targets. These targets may have high GC content, and/or strong secondary structures. The use of some chemicals, often referred as cosolvents, like the PCR Enhancing Reagent, can help reducing the melting temperature of the DNA structures, thus enabling the target amplification. 1.5 ml or 7.5 ml...

    CAD$30.00
  • CAD$40.00

    Achiever(TM) DNA Polymerase is formulated by mixing Taq and Pfu polymerases into a blended mixture, which provides the processivity of Taq along with the high fidelity of Pfu, enabling amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich...

    CAD$40.00
Showing 1 - 6 of 6 items