PCR for Difficult Targets

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Usually after a routine PCR attempt, researchers would find that their PCR has failed to amplify the intended target into the correct product. The reasons for this failure can be a few, top on the list, is the complexity of PCR target. Specifically, when the target sequence, or the primer sequence, contains high GC content >60%, or if the target sequence contains a secondary structure, the targets are difficult to denature, leading to failed PCR. The solution to this problem is to use the PCR for Difficult Targets. This type of PCR is featured by using polymerases that are better able to handle the high complexity targets, or additives such as the PCR Enhancing Reagent. 

PCR for Difficult Targets There are 2 products.

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  • CAD$30.00

    PCR Enhancing Reagent can be used to improve PCR of difficult targets. These targets may have high GC content, and/or strong secondary structures. The use of some chemicals, often referred as cosolvents, like the PCR Enhancing Reagent, can help reducing the melting temperature of the DNA structures, thus enabling the target amplification. 1.5 ml or 7.5 ml...

  • CAD$40.00

    The same high performance, with the convenience that comes with 2x master mixes. Taq Plus DNA Polymerase, a mixture of Taq and Pfu polymerases, blends the processivity of Taq with the high fidelity of Pfu. Taq Plus allows amplification of longer templates, and complex targets (e.g., GC-rich) with higher fidelity and than the single-enzyme formulations.

Showing 1 - 2 of 2 items