High Fidelity PCR

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Taq and other DNA polymerases are known to make errors in the polymerization requests of PCR. The most widely used Taq DNA polymerase is associated with high frequency of errors. For a 1 kb size PCR product, around 70% of product molecules are expected to contain on average 1 error per molecule. If your objective for a PCR is to use the products to express the gene product (protein), this type error rates are so high that either the protein sequence will be altered, or the expression level of gene product will be affected. In such a case, a high fidelity polymerase and associated buffers, magnesium and protocol for a high fidelity PCR are desirable. We introduce various products for the high fidelity PCR. 

High Fidelity PCR There are 7 products.

Showing 1 - 7 of 7 items
  • CAD$48.00

    Pfu DNA polymerase has superior thermostability and proofreading properties compared to other thermostable polymerase, with3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors, leading to high PCR fidelity. This also means that Pfu DNA polymerase-generated PCR fragments will have fewer errors...

    CAD$48.00
  • CAD$69.00

    Pfu DNA polymerase 2x master mix, the same high performance enzyme with the convenience of master mixes. The Pfu enzyme has superior thermostability and proofreading properties, enabling the correction of nucleotide-misincorporation errors, leading to high PCR fidelity. This also means that Pfu DNA polymerase-generated PCR fragments will have fewer errors...

    CAD$69.00
  • CAD$320.00

    This product is a 2X Pfu DNA Polymerase Mix, plus a separately supplied PfuDNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. This provides flexibility of optimizing Pfu DNA polymerase activity for each unique reaction and still the convenience that comes with master...

    CAD$320.00
  • CAD$100.00

    2X Achiever™ Taq Master Mix features Achiever DNA Polymerase, a mixture of Taq and Pfu polymerases, which blends the processivity of Taq with the high fidelity of Pfu. Taq Plus allows amplification of longer templates, and complex targets (e.g., GC-rich) with higher fidelity and than the single-enzyme formulations. 2X Achiever™ Taq Master Mix is also...

    CAD$100.00
  • CAD$105.00

    A proven robust PCR enzyme, GB-AMP™ HP™ PaCeR™ DNA Polymerase is suitable for all types of PCR, i.e., High Fidelity, Long PCR, high GC/complex targets, Fast PCR, Direct PCR, as well as routine PCR, you have found it in this versatile and robust PCR reagent. It was compared to Phusion (Thermo) and 8 other enzymes and found to be the best all-round PCR DNA...

    CAD$105.00
  • CAD$110.00

    GB-AMP™ PaCeR™ HP™ DNA Polymerase is a superior performance, highly flexible enzyme for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*.  This product is also capable of amplifying long fragments such as 20 kb genomic DNA and 40 kb λDNA.  View the PaCeR Brochure Here. 

    CAD$110.00
  • CAD$100.00

    2X Achiever™ Taq Master Mix features Achiever DNA Polymerase, a mixture of Taq and Pfu polymerases, which blends the processivity of Taq with the high fidelity of Pfu. Taq Plus allows amplification of longer templates, and complex targets (e.g., GC-rich) with higher fidelity and than the single-enzyme formulations. 2X Achiever™ Taq Master Mix is also...

    CAD$100.00
Showing 1 - 7 of 7 items