Mutagenesis Kits, for site-directed mutagenesis.
Mut Express II Fast Mutagenesis Kit V2 is designed for rapid site-directed mutagenesis based on ClonExpress rapid cloning technology. The amplified target fragments are digested by DpnI, cyclized by ClonExpress, and then transformed to E. coli to finish the site-directed mutagenesis.
The method works first by having primers anneal to the DNA template. Mutant strands are then synthesized with 2×TransStart® FastPfu PCR SuperMix with high fidelity. In the next step, non-mutated parental plasmid (methylated plasmid) is digested in vitro with DMT enzyme and in vivo further digested within DMT Chemically Competent Cell.
Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.